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Release date：2018-11-26

Clinical & Experimental Allergy

[IF:6.5]

Serological, genomic and structural analyses of the major mite allergen Der p 23.
doi: 10.1111/cea.12680
Background
Der p 23 was recently identified in a European population as a major allergen and potentially a chitin binding protein.
Objective
This study sought to assess the importance of Der p 23 among other Dermatophagoides allergens in a North American population and to determine the structure for functional characterization.
Methods
IgE binding to Der p 23, Der p 1, Der p 2, Der p 5, Der p 7 and Der p 8 was measured by ELISA. RNA-seq data from D. pteronyssinus were compared as estimates of allergen expression levels. The structure was analysed by X-ray crystallography and NMR.
Results
Despite a high prevalence of Der p 23, (75% vs. 87% and 79% for Der p 1 and Der p 2, respectively), the anti-Der p 23 IgE levels were relatively low. The patient response to the 6 allergens tested was variable (n = 47), but on average anti-Der p 1 and anti-Der p 2 together accounted for 85% of the specific IgE. In terms of abundance, the RNA expression level of Der p 23 is the lowest of the major allergens, thirty fold less than Der p 1 and sevenfold less than Der p 2. The structure of Der p 23 is a small, globular protein stabilized by two disulphide bonds, which is structurally related to allergens such as Blo t 12 that contain carbohydrate binding domains that bind chitin. Functional assays failed to confirm chitin binding by Der p 23.
Conclusions and Clinical Relevance
Der p 23 accounts for a small percentage of the IgE response to mite allergens, which is dominated by Der p 1 and Der p 2. The prevalence and amount of specific IgE to Der p 23 and Der p 2 are disproportionately high compared to the expression of other Dermatophagoides allergens.
All Authors:
G A. Mueller1 , T. A. Randall2 , J. Glesner3 , L. C. Pedersen1 , L. Perera1 , L. L. Edwards1 , E. F. DeRose1 , M. D. Chapman3 , R. E. London1 and A. Pomes3 1 Genome Integrity and Structural Biology Laboratory, Research Triangle Park, NC, USA, 2 Integrative Bioinformatics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA and 3 INDOOR Biotechnologies, Inc., Charlottesville, VA, USA

2018-11-10 Article